| 质粒名称: | pAM PAT-ProTRY GW |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79755 |
| 存储实验室: | Nico Dissmeyer, Martin Hulskamp |
|
载体骨架基本信息 |
|
| 载体名称: | Binary vector pAMPAT-MCS |
| 完整质粒大小: | 7803bp |
| 载体类型: | Plant Expression |
| 筛选标记: | Basta |
| 载体抗性: | Chloramphenicol and Ampicillin |
| 生长温度: | 37 ℃ |
| 宿主菌: | ccdB Survival |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 启动子: | ProTRY |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | gaccggcaacaggattcaatcttaag |
| 3’测序引物 | GAATTAGAAATTTTATTGATAGAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pAM PAT-ProTRY GW was a gift from Nico Dissmeyer & Martin Hulskamp (Addgene plasmid # 79755 ; http://n2t.net/addgene:79755 ; RRID:Addgene_79755) |
| 参考文献部分: | Mutual control of intracellular localisation of the patterning proteins AtMYC1, GL1 and TRY/CPC in Arabidopsis. Pesch M, Schultheiss I, Digiuni S, Uhrig JF, Hulskamp M. Development. 2013 Aug;140(16):3456-67. doi: 10.1242/dev.094698. 10.1242/dev.094698 PubMed 23900543 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAM PAT-ProTRY GW 7803 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION binary DEST vector for plant expression.
ACCESSION .
VERSION .
KEYWORDS pAM PAT-ProTRY GW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7803)
AUTHORS Pesch M, Schultheiss I, Digiuni S, Uhrig JF, Hulskamp M
TITLE Mutual control of intracellular localisation of the patterning
proteins AtMYC1, GL1 and TRY/CPC in Arabidopsis.
JOURNAL Development. 2013 Aug;140(16):3456-67. doi: 10.1242/dev.094698.
PUBMED 23900543
REFERENCE 2 (bases 1 to 7803)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7803
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 2..26
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
terminator 120..372
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(392..943)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(985..1164)
/label=NOS promoter
/note="nopaline synthase promoter"
protein_bind 2788..2912
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2937..2967
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 3021..3680
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(3087..3106)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 4022..4327
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4206..4225
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(4368..4492)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 4863..4887
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 4999..5708
/label=oriV
/note="incP origin of replication"
promoter 5782..5873
/gene="bla"
/label=AmpR promoter
CDS 5874..6734
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6092..6111)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6905..7493
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7394..7413
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
61 ggacgttttt aatgatcgaa tactaacgtc tctaccagat atcagcttgc atgccggtcg
121 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
181 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
241 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
301 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
361 gtttgaacga tctgcttgac tctaggggtc atcagatttc ggtgacgggc aggaccggac
421 ggggcggcac cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt
481 gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc
541 gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct
601 ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg
661 gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccagggac
721 ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct
781 cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt
841 tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct
901 cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat cccctcgatc
961 gagttgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
1021 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
1081 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca
1141 gtggctcctt caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc
1201 atcggcgggg gtcataacgt gactccctta attctccgct catgatcaga ttgtcgtttc
1261 ccgccttcgg tttgggcgcg cctcttagct tcgaagaaaa cgcaaatcag ggattttggt
1321 tgtatcatgt gtgagagatt gaatttctcc agaagcactc attaaatcgc gtgtgtaagg
1381 agttgccata cagcgtcgta tgtaatgtct tagtcactgt tctagaatta aaacatatgt
1441 caaatgtaga attaaaaaac aggggttaaa atgttaaatt attcggagaa gtggaattat
1501 tatattaaaa acattgtgtg attttgggat aaaatctgaa tcatgttttt tattgtgcca
1561 aaaaatttct gtttcaacct cttcgtggag atgaaagaaa agtcaatcgg gaatgagact
1621 tcttgtataa cgaatgaaaa tatgaacatg ttgattaatg aattaattaa catacgtact
1681 atattaagtg ggaatattag gtataggcct tcgattttcc gttttgtttg gttttttcga
1741 ttttaataat atttctctaa attaaaataa attttagttt ggtttattta aaatattaat
1801 aaaataatat aaataactat ctaattaata tattaaaaag tattttctat atatattgat
1861 ataaatatta ttcccatgtt ttatattggg tgacgttatc ttttaagtaa agttgtaaga
1921 tatctaactt attcctacat tataataact atcaaaataa aataactaat tgtagtgaca
1981 atgatgacta tcaaaatatg tatgtgtttt ggctcaaaca tagcgtttaa taacaaacac
2041 aatggacaat tttaataaat ttgatcgatt agattttgtt tcattttcat gcactagatt
2101 aaggttcgaa ttagattcat tttagtgtat atagtgaaac taatttgtta ctatatggaa
2161 aaaaaaataa gtttattttt ggttcaagaa tttttattcg gtacggtttg gtgtgtatgt
2221 ccaaccctga aaaaaaaact tgaaaaacta ataacctaat aacaactaat tttcttagaa
2281 atacaaattg gacttgaata caaaaatgga aagaacaaca acaaatcaat aactaaaaca
2341 tcgttgaact tgcatctgcc aaataaaaat caataaatcc cattattcta aaaagatagt
2401 aggtttagaa aaattgaata aatggaaaaa aaaaattgtt aggatctacg aatgagaaca
2461 aagttagcct tcaaaatact tacaaatccc aataaaagac ttcatctcca tgtgtatttg
2521 agtgtcaacg acaagtctac acaaagggta agaggtcaac aagaccacac aacacttctt
2581 actattagtt ttgcaaaggc cgttcgttgg acatttcctt ctctctcctc ccctcttctt
2641 cttcttgttc gctctataaa ctctcatctc tcacgtcttt ttttccttac attctccaaa
2701 ctcaaaattt catcacatta atttctctct atttttcttt tcttacttca atagtcgacc
2761 tcgagaaaga ggatccacct gaggatcaca agtttgtaca aaaaagctga acgagaaacg
2821 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
2881 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
2941 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
3001 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
3061 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
3121 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
3181 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
3241 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
3301 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
3361 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
3421 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
3481 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
3541 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
3601 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
3661 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg
3721 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
3781 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
3841 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
3901 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
3961 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga
4021 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
4081 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
4141 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
4201 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
4261 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
4321 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
4381 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
4441 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatcctcc
4501 gttccatggg ctagaagctt gttgagaatt catcgcccgg gactgggtta acttccgatc
4561 gattctagac tagttctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc
4621 tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt
4681 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
4741 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga ccgggcggcc
4801 gccaccgcgg tggaggggga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg
4861 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
4921 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgt acatcaccga
4981 cgagcaaggc aagaccgagc gcctttccga cgctcaccgg gctggttgcc ctcgccgctg
5041 ggctggcggc cgtctatggc cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga
5101 gacaccggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
5161 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
5221 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
5281 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
5341 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
5401 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
5461 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
5521 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
5581 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
5641 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
5701 aatggcagcg ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat
5761 cagcccgaca agctacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
5821 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
5881 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
5941 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
6001 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
6061 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
6121 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
6181 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
6241 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
6301 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
6361 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
6421 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
6481 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
6541 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
6601 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
6661 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
6721 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
6781 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
6841 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
6901 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
6961 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
7021 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
7081 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
7141 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
7201 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
7261 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
7321 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
7381 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
7441 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
7501 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggactcta
7561 gctagaggat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
7621 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
7681 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
7741 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
7801 ctg
//
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